Michigan Traditional Matchmaking boutique of Janis Spindel and Carly Spindel

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RNA structure is intimately related to function, yet methods to identify base-paired RNA strands in a transcriptome-wide manner in cells have remained elusive. One recent paper in Cell and two in Molecular Cell describe related methods to identify RNA sequences that interact in living cells, setting the stage for break-throughs in our understanding of RNA structure and function. Just like proteins, RNA molecules adopt 3D structures that are important for their processing and function.

In fact, nearly all textbooks depict messenger RNAs as linear, non-structured, single-stranded molecules. Not only do most RNAs likely have specific 3D structures, but these structures are almost certainly important for the functions of these RNAs. Over the past several decades, a large toolbox of diverse methods has been developed to characterize RNA secondary structures and identify RNA sequences that base-pair with one another, whether in cis or in trans.

These include computational modeling, phylogenetic analysis, genetic testing, and biochemical probing reviewed in Wan et al. For example, several computational tools exist that use thermodynamic rules to predict RNA secondary structure based on the primary sequence. Comparative analysis of orthologous sequences from multiple species can also provide evidence supporting RNA secondary structures.

Specifically, co-variation of distantly located bases is indicative that those nucleotides base-pair with one another. Similarly, unpaired sequences often exhibit more rapid and less-constrained sequence divergence than base-paired sequences. Traditional genetic experimentation can be used to test and validate secondary structure predictions.

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RNA Matchmaking: Finding Cellular Pairing Partners

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LastFirst is a bespoke matchmaking club headquartered in Manhattan, serving clients in cosmopolitan cities worldwide. Founded in , LastFirst has carved out a unique space in the industry by combining the personalized approach of a boutique brand with the structure and resourcing of a larger company. Our high satisfaction rate is due primarily to our selectivity.

We work with a very small number of clients, using proactive targeted networking, plus searches of our existing membership database, to match each one. We are inclusive of all genders, sexual orientations, races and ethnicities. The common threads among our accepted clients are more nuanced: high moral character, real desire to be in a relationship, an interesting life story, and attractive physical appearance, to name a few.

In the app-ocalypse of Tinder, Bumble, Raya and beyond, LastFirst offers a luxury alternative to make dating less random and more meaningful. Founder Emily Holmes Hahn combines her sophisticated social networks and innate way of connecting with friends and strangers alike to shape the LastFirst brand. She founded LastFirst as an independent consultancy, and thoughtfully scaled the business, always prioritizing the client experience.

Emily currently drives our West Coast and Europe membership expansions, and takes a front seat role as matchmaker for several New York accounts too. Emily enjoys strong coffees and boutique champagnes often in the same sitting , and loves any occasion she can dress for.

Patents Matchmaking Formula

This application claims the benefit under 35 U. Provisional Application No. Aspects of the present invention are directed generally to methods and systems for matching users in an online gaming environment. More particularly aspects of the present disclosure are directed to methods and systems for matching suitable users in an interactive online environment based upon hardware parameters of the computing systems of each user.

Online gaming has become a form of entertainment for millions of people. Accessibility to gaming systems allows a large number of users to connect online and interact with others.

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We use a scientific approach to partnership and courtship that supports you in your search for your better half, saving you time and energy by selecting only suitable candidates. Our goal is to find our customers the love of their lives and when you join, you will see that others in the network are looking for the same kind of relationship you are.

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